Purpose
Phase-variance optical coherence tomography (PV-OCT) provides volumetric imaging of
the retinal vasculature without the need for intravenous injection of a fluorophore.
We compare images from PV-OCT and fluorescein angiography (FA) for normal individuals
and patients with age-related macular degeneration (AMD) and diabetic retinopathy.
Design
This is an evaluation of a diagnostic technology.
Participants
Four patients underwent comparative retinovascular imaging using FA and PV-OCT. Imaging
was performed on 1 normal individual, 1 patient with dry AMD, 1 patient with exudative
AMD, and 1 patient with nonproliferative diabetic retinopathy.
Methods
Fluorescein angiography imaging was performed using a Topcon Corp (Tokyo, Japan) (TRC-50IX)
camera with a resolution of 1280 (H) × 1024 (V) pixels. The PV-OCT images were generated
by software data processing of the entire cross-sectional image from consecutively
acquired B-scans. Bulk axial motion was calculated and corrected for each transverse
location, reducing the phase noise introduced from eye motion. Phase variance was
calculated through the variance of the motion-corrected phase changes acquired within
multiple B-scans at the same position. Repeating these calculations over the entire
volumetric scan produced a 3-dimensional PV-OCT representation of the vasculature.
Main Outcome Measures
Feasibility of rendering retinal and choroidal microvasculature using PV-OCT was compared
qualitatively with FA, the current gold standard for retinovascular imaging.
Results
Phase-variance OCT noninvasively rendered a 2-dimensional depth color-coded vasculature
map of the retinal and choroidal vasculature. The choriocapillaris was imaged with
better resolution of microvascular detail using PV-OCT. Areas of geographic atrophy
and choroidal neovascularization imaged by FA were depicted by PV-OCT. Regions of
capillary nonperfusion from diabetic retinopathy were shown by both imaging techniques;
there was not complete correspondence between microaneurysms shown on FA and PV-OCT
images.
Conclusions
Phase-variance OCT yields high-resolution imaging of the retinal and choroidal microvasculature
that compares favorably with FA.
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Article Info
Publication History
Published online: October 22, 2013
Accepted:
September 4,
2013
Received in revised form:
August 30,
2013
Received:
March 27,
2013
Footnotes
Financial Disclosure(s): The author(s) have made the following disclosure(s): D.M.S., J.F., and S.E.F. hold an issued patent on the PV-OCT technology. They also hold founders' shares in a company with an interest in the technology. The remaining authors have no conflict of interest.
Supported by the National Eye Institute ( EY 014743 ), Research to Prevent Blindness , Beckman Institute , That Man May See Foundation , and Howard Hughes Medical Institute Med-into-Grad Initiative ( 56006769 ). The sponsors and funding agencies had no role in the design or conduct of this research.
Identification
Copyright
© 2014 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved.
